AbOliGo Closes Oversubscribed Round to Accelerate Multiomic Research Tools and Appoints Proven Life Sciences Board CAMBRIDGE, UK – August, 2025 – AbOliGo , a biotechnology company pioneering high-performance antibody-oligonucleotide conjugates (AOCs), today announced the successful close of an oversubscribed seed ... 6 Aug 2025 News
Compatibility of Phosphorothioate-Stabilised Oligonucleotides with Maleimide Chemistry Protein–DNA conjugates play essential roles in biotechnology, diagnostics, and molecular imaging. However, DNA-based constructs are susceptible to nuclease degradation, which might limit their effecti... 1 Aug 2025 Knowledge Hub
How to Eliminate Non Specific Binding of Antibody Oligo Conjugates Antibody–oligonucleotide conjugates have emerged as powerful tools for highly multiplexed and amplified protein detection in imaging and sequencing workflows. Yet when single‑stranded DNA (ssDNA) is c... 24 Jul 2025 Knowledge Hub
Proximity Ligation Assays for Analyzing Protein - Protein Interactions. Introduction to Proximity Ligation Assays (PLA) Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly ... 20 Jun 2025 Knowledge Hub
Approaches for making antibody-oligonucleotide conjugates Antibody-oligonucleotide conjugates (AOCs) may be synthesized using a wide range of chemical approaches. These include stochastic methods where protein lysines provide a large number of potential site... 29 May 2025 Knowledge Hub
Bioconjugation: Tips, Tricks, and Commentary Part 2 Written by Nick Gee - Conjugation Expert Part 2 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? In part 1, I di... 21 May 2025 Knowledge Hub
Bioconjugation: Tips, Tricks, and Commentary - part 1 Written by Nick Gee - Conjugation Expert Part 1 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? The degree of l... 21 May 2025 Knowledge Hub
Flexible Multiplexed Imaging via Cyclic Immunofluorescence with DNA-Barcoded Antibodies Introduction: Addressing the Limitations of Multiplex Imaging Advances in spatial proteomics have underscored the need for highly multiplexed imaging platforms that can preserve tissue integrity while... 21 May 2025 Knowledge Hub
Application of capillary zone electrophoresis for the characterization of antibody-oligo conjugates Analytical tests that can be utilized for the characterization of antibody-oligonucleotide complexes (AOCs) include gel exclusion chromatography, SDS-PAGE, mass spectrometry, and capillary electrophor... 29 Apr 2025 Knowledge Hub
IN-DEPTH: IN-situ DEtailed Phenotyping To High Resolution IN-DEPTH: IN -situ DE tailed P henotyping to H igh Resolution Introduction In the evolving field of biomarker discovery and spatial biology, IN -situ DE tailed P henotyping T o Hi gh Resolution (IN-DE... 8 Apr 2025 Knowledge Hub
Site-directed antibody conjugation methods 1: application of transglutaminase in the production of antibody-oligo conjugates Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic en... 2 Apr 2025 Knowledge Hub
Degree of Labeling Impacts AOCs for Single-Cell Multi-Omics Degree of Labeling Impacts AOCs for Single-Cell Multi-Omics If you’ve ever tried single-cell RNA sequencing (scRNA-seq), you know the struggle, transcriptomics alone doesn’t always give the full pictu... 25 Mar 2025 Knowledge Hub