Proximity Ligation Assays for Analyzing Protein - Protein Interactions. Introduction to Proximity Ligation Assays (PLA) Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly ... 20 Jun 2025
Approaches for making antibody-oligonucleotide conjugates Antibody-oligonucleotide conjugates (AOCs) may be synthesized using a wide range of chemical approaches. These include stochastic methods where protein lysines provide a large number of potential site... 29 May 2025
Bioconjugation: Tips, Tricks, and Commentary Part 2 Written by Nick Gee - Conjugation Expert Part 2 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? In part 1, I di... 21 May 2025
Bioconjugation: Tips, Tricks, and Commentary - part 1 Written by Nick Gee - Conjugation Expert Part 1 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? The degree of l... 21 May 2025
Flexible Multiplexed Imaging via Cyclic Immunofluorescence with DNA-Barcoded Antibodies Introduction: Addressing the Limitations of Multiplex Imaging Advances in spatial proteomics have underscored the need for highly multiplexed imaging platforms that can preserve tissue integrity while... 21 May 2025
Application of capillary zone electrophoresis for the characterization of antibody-oligo conjugates Analytical tests that can be utilized for the characterization of antibody-oligonucleotide complexes (AOCs) include gel exclusion chromatography, SDS-PAGE, mass spectrometry, and capillary electrophor... 29 Apr 2025
IN-DEPTH: IN-situ DEtailed Phenotyping To High Resolution IN-DEPTH: IN -situ DE tailed P henotyping to H igh Resolution Introduction In the evolving field of biomarker discovery and spatial biology, IN -situ DE tailed P henotyping T o Hi gh Resolution (IN-DE... 8 Apr 2025
Site-directed antibody conjugation methods 1: application of transglutaminase in the production of antibody-oligo conjugates Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic en... 2 Apr 2025
Degree of Labeling Impacts AOCs for Single-Cell Multi-Omics Degree of Labeling Impacts AOCs for Single-Cell Multi-Omics If you’ve ever tried single-cell RNA sequencing (scRNA-seq), you know the struggle, transcriptomics alone doesn’t always give the full pictu... 25 Mar 2025
Scientific Techniques Using Antibody-Oligonucleotide Conjugates Antibody-Oligonucleotide Conjugate Technology Development Antibody-oligonucleotide conjugation has revolutionized molecular detection since the 1992 introduction of Immuno-PCR, achieving femtomolar se... 12 Mar 2025
Achieving batch-to-batch consistency with Antibody Oligonucleotide Conjugates (AOCs) Batch-to-batch consistency in antibody conjugate production is challenging with any type of label and can only be achieved with tight control of multiple reaction parameters. While the degree of label... 4 Mar 2025
Smart application of an old conjugation reagent in the production of AOCs Antibody-oligonucleotide conjugates (AOCs) are used in techniques such as immuno-PCR, proximity ligation assay (PLA) and proximity extension assay (PEA), and are also being examined as potential thera... 25 Feb 2025