Use of site-specific antibody-oligo conjugates in microarray capture applications Conventional microarrays are made by adsorbing antibodies onto hydrophobic or chemically reactive solid surfaces, which can lead to denaturation, random orientation, loss of binding activity and reduc... 28 Aug 2025
Amplifying Discovery: How Antibody–Oligo Conjugates Power SABER-IMC Introduction Imaging Mass Cytometry (IMC) has the potential to profile ~40 proteins in tissues but struggles to detect low-abundance markers. The latest advance—SABER-IMC ( S ignal A mplification B y ... 19 Aug 2025
Compatibility of Phosphorothioate-Stabilised Oligonucleotides with Maleimide Chemistry Protein–DNA conjugates play essential roles in biotechnology, diagnostics, and molecular imaging. However, DNA-based constructs are susceptible to nuclease degradation, which might limit their effecti... 1 Aug 2025
How to Eliminate Non Specific Binding of Antibody Oligo Conjugates Antibody–oligonucleotide conjugates have emerged as powerful tools for highly multiplexed and amplified protein detection in imaging and sequencing workflows. Yet when single‑stranded DNA (ssDNA) is c... 24 Jul 2025
Proximity Ligation Assays for Analyzing Protein - Protein Interactions. Introduction to Proximity Ligation Assays (PLA) Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly ... 20 Jun 2025
Approaches for making antibody-oligonucleotide conjugates Antibody-oligonucleotide conjugates (AOCs) may be synthesized using a wide range of chemical approaches. These include stochastic methods where protein lysines provide a large number of potential site... 29 May 2025
Bioconjugation: Tips, Tricks, and Commentary Part 2 Written by Nick Gee - Conjugation Expert Part 2 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? In part 1, I di... 21 May 2025
Bioconjugation: Tips, Tricks, and Commentary - part 1 Written by Nick Gee - Conjugation Expert Part 1 of 2: Random conjugation of labels to antibody lysines—is the concern over the impact of CDR modification on antigen binding overstated? The degree of l... 21 May 2025
Flexible Multiplexed Imaging via Cyclic Immunofluorescence with DNA-Barcoded Antibodies Introduction: Addressing the Limitations of Multiplex Imaging Advances in spatial proteomics have underscored the need for highly multiplexed imaging platforms that can preserve tissue integrity while... 21 May 2025
Application of capillary zone electrophoresis for the characterization of antibody-oligo conjugates Analytical tests that can be utilized for the characterization of antibody-oligonucleotide complexes (AOCs) include gel exclusion chromatography, SDS-PAGE, mass spectrometry, and capillary electrophor... 29 Apr 2025
IN-DEPTH: IN-situ DEtailed Phenotyping To High Resolution IN-DEPTH: IN -situ DE tailed P henotyping to H igh Resolution Introduction In the evolving field of biomarker discovery and spatial biology, IN -situ DE tailed P henotyping T o Hi gh Resolution (IN-DE... 8 Apr 2025
Site-directed antibody conjugation methods 1: application of transglutaminase in the production of antibody-oligo conjugates Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic en... 2 Apr 2025