9 articles • Comprehensive approaches to antibody-oligonucleotide conjugation
Explore comprehensive approaches to antibody-oligonucleotide conjugation, including site-specific methods, chemical compatibility studies, and optimization strategies for various conjugation chemistries.
Protein–DNA conjugates play essential roles in biotechnology, diagnostics, and molecular imaging. However, DNA-based constructs are susceptible to nuclease degradation, which might limit their effectiveness in biological applications.
Antibody-oligonucleotide conjugates (AOCs) may be synthesized using a wide range of chemical approaches. These include stochastic methods where protein lysines provide a large number of potential sites for conjugation.
Transglutaminase catalyses the formation of an isopeptide bond between a lysine (K) residue and the γ-carboxamide group of a glutamine (Q) residue. The microbial (mTGase) rather than the eukaryotic enzyme is typically used.
Conventional microarrays are made by adsorbing antibodies onto hydrophobic or chemically reactive solid surfaces, which can lead to denaturation, random orientation, loss of binding activity and reduced sensitivity.
Innovative use of disuccinimidyl suberate (DSS) for cost-effective conjugation at µg scale, demonstrating how established reagents can be repurposed for modern applications.
Optimization strategies for single and double-stranded oligonucleotide conjugation, including buffer conditions, reaction parameters, and purification methods.
Learn about BA-PFP2 reagent for creating hydrolytically stable, site-specific antibody-oligonucleotide conjugates with improved stability over maleimide chemistry.
Comprehensive comparison of random and site-specific antibody conjugation methods for therapeutic applications, including pros, cons, and manufacturing considerations.
Comparing site-specific vs random conjugation chemistries for AOCs in cyclic immunofluorescence applications. Comprehensive guide to conjugation methods and signal removal strategies.
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