Knowledge Hub

Conjugation Chemistry & Methods

Comprehensive approaches to antibody-oligonucleotide conjugation, including site-specific methods, chemical compatibility, and optimization strategies.

• • Site-Specific vs Random Antibody Conjugation for Therapeutic Market
• • Use of Benzoylacrylic-labelled Oligonucleotides for Site-specific Conjugates
• + 2 more articles

Bioconjugation Optimization & Troubleshooting

Expert insights, best practices, and troubleshooting strategies for successful bioconjugation reactions and eliminating non-specific binding.

• • Bioconjugation: Tips, Tricks, and Commentary - Part 1
• • Bioconjugation: Tips, Tricks, and Commentary - Part 2
• + 2 more articles

Analytical Characterization & Quality Control

Methods for conjugate quality assessment, degree of labeling optimization, and achieving batch-to-batch consistency in AOC manufacturing.

• • A Guide to Using SDS-PAGE to Characterize Antibody-Oligonucleotide Conjugates
• • Application of Capillary Zone Electrophoresis for AOC Characterization

Single-Cell & Multi-Omics Applications

Protocols and optimization strategies for single-cell protein detection, staining optimization, and multi-omics workflows.

• • Should We Barcode All Research Antibodies with Oligos?
• • Optimizing Antibody Oligo-Conjugates Staining for Single-Cell Multiomics
• + 1 more articles

Protein Interaction & Proximity Assays

Applications in protein interaction studies and proximity detection using Proximity Ligation Assays (PLA) and related techniques.

• • Proximity Ligation Assays for Analyzing Protein-Protein Interactions

Advanced Imaging & Multiplexing

Revolutionary imaging techniques using DNA-barcoded conjugates, signal amplification methods, and high-resolution spatial analysis.

• • AOC Techniques Continue to Advance: Solving Nuclear Imaging with Left-Handed DNA
• • Flexible Multiplexed Imaging via Cyclic Immunofluorescence with DNA-Barcoded Antibodies
• + 2 more articles